Author(s) :

M Zaigham Javed, Nimra khan, Azeem Haider, M Kaif, Sharmeen Zulfiqar.

Volume/Issue :

Abstract :

Next-generation sequencing is an innovative method of recognizing genetic mutations and changes in the sequencing of DNA or RNA. Next-generation sequencing generates large amounts of sequence data faster than the traditional Sanger sequencing approach. It is performed in various steps, including DNA fragmentation, Gene Library, Sequencing, and Data analysis. Second-generation sequencing includes Pyrosequencing and Ion torrent sequencing available for clinical diagnosis. Nanopore sequencing is the fourth hybrid NGS and does not require PCR amplification. NGS is widely used to identify inherited genetic disorders, novel cancer mutations, drug resistance, HLA typing, and sequencing of meta-genomics. It is concluded that NGS has changed the landscape in clinical settings and research studies. Using this technique, a lot of complex sequencing is reachable that was not attainable with traditional methods. Implementing NGS in clinical settings will enhance healthcare outcomes and improve patient care.

No. of Downloads :

1