Isolation of Effective Plant Growth-Promoting Rhizobacteria (PGPR) to Construct Synthetic Communities and Evaluate Their Impact on Maize (Zea mays)
Publication Date : 31-07-2025
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Abstract :
The overutilization of artificial fertilizers in maize (Zea mays) cultivation has cause to environmental degradation and decrease soil microbial diversity. This study intended to isolate effective Plant Growth-Promoting Rhizobacteria (PGPR) from the maize rhizosphere, construct synthetic communities, and assess their impact on plant growth under glasshouse conditions. Rhizobacteria were isolated and screened using high-throughput microplate assays for phosphate solubilization, indole-3-acetic acid production, and ammonia generation. Genetic diversity and compatibility were assessed using BOX-PCR fingerprinting. Synthetic communities were formulated with compatible, functionally complementary strains and applied to maize in a pot trial alongside controls. Considerable improvements were observed in shoot and root length, biomass, chlorophyll content, and yield factors. Soil biological markers such as microbial biomass carbon and enzyme activities also enhanced. These Results emphasize the capability of PGPR consortia as biofertilizers to long term increase maize productivity and soil health.
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