Articles

Indus River is the largest river of Pakistan that originated from Tibetan Plateau of Gilgit-Baltistan and flow through the entire Pakistan before merging with Arabian Sea in District Sindh. This river is hot spot of biodiversity having more than 180 fresh water fish species. In this study a total of 10 fish samples were collected from the selected regions i.e., Ghazi, Hund, Beka, and Kund Park from Indus River district Swabi, Khyber Pakhtunkhwa, Pakistan from 24 May to 28 August 2024. The collected fish samples were identified morphologically as Cirrhinus mrigala through standard fish keys and preserved in 100% ethanol for the purpose of DNA extraction. The DNA were extracted from fish muscle and liver using Phenol-Chloroform protocol and characterized by using universal genetic marker Cytochrome Oxidase sub unit I (COI) gene. The new available sequence were submitted into GenBank for accession number PV562800. The phylogenetic analysis of the new reported sequence of C. mrigala showed 100% similarity with other reported similar species from India, China, France and Bangladesh. Fish fauna of Indus River decline day by day due to over fishing, pollution and habitat loss. This study provide a baseline for the management of fish fauna inhibiting in River Indus.